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Opto Engine LLC
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IkeCool Corporation
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NEWDOON Inc
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Laserline GmbH
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IkeCool Corporation
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NanoSight ltd
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Becton Dickinson
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Image Search Results
Journal: Nature Communications
Article Title: Distinct medial amygdala oxytocin receptor neurons projections respectively control consolation or aggression in male mandarin voles
doi: 10.1038/s41467-024-51652-8
Figure Lengend Snippet: a , b Virus regimen (adapted from The Mouse Brain in Stereotaxic Coordinates by Paxinos and Franklin, a ) and schedule ( b ) for optogenetic activation of the somata. c , d Images of OXTR-hCHR2 expression (mCherry, red) in the MeA OXTR+AI and MeA OXTR+VMHvl (scale bars, 500 μm). 9 and 8 independent repetitions with similar results in ( c ) and ( d ). e Overlapped images of hCHR2 (mCherry) and anti-OXTR (AF488, green) cells in the MeA OXTR+AI and MeA OXTR+VMHvl (scale bars, 50 μm). n = 3 voles/group. f Representative trace from electrophysiological recordings showing photoactivation of the MeA OXTR+AI or MeA OXTR+VMHvl soma. g – k Comparison of allogrooming and sniffing siblings, and of attacking intruders between the AI-retro CHR2 and mCherry groups. n (mCherry) = 6 voles; n (CHR2) = 9 voles. Allogrooming frequency: CHR2-Light OFF versus CHR2-Light ON, p < 0.001; mCherry-Light ON versus CHR2-Light ON, p = 0.008; Allogrooming time proportion: CHR2-Light OFF versus CHR2-Light ON, p = 0.026; mCherry-Light ON versus CHR2-Light ON, p = 0.026; sniffing frequency: CHR2-Light OFF versus CHR2-Light ON, p = 0.040; mCherry-Light ON versus CHR2-Light ON, p = 0.027. l – p Comparison of allogrooming and sniffing siblings, and of attacking intruders between the VMHvl-retro CHR2 and mCherry groups. n (mCherry) = 6 voles, n (CHR2) = 8 voles. Sniffing frequency: mCherry-Light ON versus CHR2-Light ON, p < 0.001; attack time proportion: CHR2-Light OFF versus CHR2-Light ON, p = 0.002; attack frequency: CHR2-Light OFF versus CHR2-Light ON, p = 0.002. Data was analyzed by repeated measure two-way ANOVA with Sidak’s multiple comparison test ( g – p ). *** p < 0.001, ** p < 0.01, * p < 0.05. Data are presented as the means +/− SEM. Statistical details are presented in Supplementary Data. file. Source data are provided as a Source Data file.
Article Snippet: Ferrules were connected (by patch cords) to a 473-nm laser diode of the
Techniques: Virus, Activation Assay, Expressing, Comparison
Journal: Nature Communications
Article Title: Distinct medial amygdala oxytocin receptor neurons projections respectively control consolation or aggression in male mandarin voles
doi: 10.1038/s41467-024-51652-8
Figure Lengend Snippet: a , b Virus regimen (adapted from The Mouse Brain in Stereotaxic Coordinates by Paxinos and Franklin, a ) and schedule ( b ) for optogenetic activation of the fibers. c , d Images of OXTR-hCHR2 expression (mCherry, red) in MeA OXTR+AI and MeA OXTR+VMHvl (scale bars, 500 μm). 7 independent repetitions with similar results in ( c ) and ( d ). e Overlapped images of hCHR2 (mCherry) and anti-OXTR (AF488, green) cells in the MeA OXTR+AI and MeA OXTR+VMHvl (scale bars, 50 μm). n = 3 voles/group. f Representative traces from electrophysiological recordings showing photoactivation of the MeA OXTR+AI or MeA OXTR+VMHvl somata. g – k Comparison of allogrooming and sniffing siblings, and of attacking intruders between the AI-retro CHR2 and mCherry groups. n (mCherry) = 6 voles; n (CHR2) = 7 voles. In g – k , allogrooming frequency: CHR2-Light OFF versus CHR2-Light ON, p = 0.0195; mCherry-Light ON versus CHR2-Light ON, p = 0.0158; allogrooming time proportion: CHR2-Light OFF versus CHR2-Light ON, p = 0.004; mCherry-Light ON versus CHR2-Light ON, p = 0.031; sniffing frequency: CHR2-Light OFF versus CHR2-Light ON, p = 0.0375. l – p Comparison of allogrooming and sniffing siblings, and of attacking intruders between the VMHvl-retro CHR2 and mCherry groups. n (mCherry) = 6 voles, n (CHR2) = 7 voles. In l – p , attack time proportion: CHR2-Light OFF versus CHR2-Light ON, p = 0.006; attack frequency: CHR2-Light OFF versus CHR2-Light ON, p = 0.0321. Data was analyzed by Repeated measure two-way ANOVA with Sidak’s multiple comparison test ( g – p ). *** p < 0.001, ** p < 0.01, * p < 0.05. Data are presented as the means +/− SEM. Statistical details are presented in Supplementary Data. 1 file. Source data are provided as a Source Data file.
Article Snippet: Ferrules were connected (by patch cords) to a 473-nm laser diode of the
Techniques: Virus, Activation Assay, Expressing, Comparison
Journal: Nature Communications
Article Title: Distinct medial amygdala oxytocin receptor neurons projections respectively control consolation or aggression in male mandarin voles
doi: 10.1038/s41467-024-51652-8
Figure Lengend Snippet: a, b Virus regimen (adapted from The Mouse Brain in Stereotaxic Coordinates by Paxinos and Franklin, a ) and schedule ( b ) for optogenetic activation of the glutamatergic (VGlut2) fibers of the MeA OXTR+AI and MeA OXTR+VMHvl . c , d Images of VGlut2-hCHR2 expression (mCherry, red) in MeA OXTR+AI and MeA OXTR+VMHvl . Scale bars, 500 μm. 7 independent repetitions with similar results in ( c ) and ( d ). e Representative overlapped images of VGlut2-hCHR2 somata (mCherry) and anti-OXTR (AF488, green) cells. Scale bars, 50 μm. n = 3 voles/group. f Representative traces from electrophysiological recordings showing photoactivation of the glutamatergic MeA OXTR+AI or MeA OXTR+VMHvl somata. g – k Comparison of allogrooming and sniffing siblings, and of attacking intruders between the AI-retro VGlut2-CHR2 and VGlut2-mCherry groups. n (mCherry) = 7 voles; n (CHR2) = 7 voles. Allogrooming frequency: CHR2-Light OFF versus CHR2-Light ON, p = 0.0041; mCherry-Light ON versus CHR2-Light ON, p = 0.0406; Allogrooming time proportion: CHR2-Light OFF versus CHR2-Light ON, p = 0.038; mCherry-Light ON versus CHR2-Light ON, p = 0.032; Sniffing frequency: CHR2-Light OFF versus CHR2-Light ON, p = 0.006; mCherry-Light ON versus CHR2-Light ON, p = 0.025. l – p Comparison of allogrooming and sniffing siblings, and of attacking intruders between the VMHvl-retro VGlut2-CHR2 and VGlut2-mCherry groups. n (mCherry) = 6 voles, n (CHR2) = 7 voles. Attack time proportion: CHR2-Light OFF versus CHR2-Light ON, p = 0.003; mCherry-Light ON versus CHR2-Light ON, p = 0.008; Attack frequency: CHR2-Light OFF versus CHR2-Light ON, p = 0.002; mCherry-Light ON versus CHR2-Light ON, p = 0.004. Data was analyzed by repeated measure two-way ANOVA with Sidak’s multiple comparison test ( g – p ). *** p < 0.001, ** p < 0.01, * p < 0.05. Data are presented as the means +/− SEM. Statistical details are presented in the Supplementary Data file. Source data are provided as a Source Data file. VGlut2 vesicular glutamate transporter 2.
Article Snippet: Ferrules were connected (by patch cords) to a 473-nm laser diode of the
Techniques: Virus, Activation Assay, Expressing, Comparison